Lyophilizing Foot-and Mouth Disease Virus at Low Drying Temperature

Foot-and-mouth disease virus: stability of neutralizing antibody after freeze-drying and air-drying.

It is possible to stabilize foot-and-mouth disease antiserum by either freeze-drying in bottles or air-drying on paper for periods of 7 months.

Foot-and-mouth disease virus.

Foot-and-mouth disease virus (FMDV) is an aphthovirus of the family Picornaviridae and the etiological agent of the economically most important animal disease. As a typical picornavirus, FMD virions are nonenveloped particles of icosahedral symmetry and its genome is a single stranded RNA of about 8500 nucleotides and of positive polarity. FMDV RNA is infectious and it replicates via a compleme...

Freeze-drying of foot and mouth disease virus and its application in inactivated virus vaccine production

Five different subtypes of foot and mouth disease (FMD) virus propagated in BHK21 C13 monolayer cell cultures, namely 05, A5/!0, A22, C¡ and Asia 1/1, were used to study the stability of live virus during freeze-drying and subsequent storage. Binary ethyleneimine-inactivated FMD virus vaccines adjuvanted with purified saponin, "Quil-A", without aluminium hydroxide gel, were examined before and ...

Freeze-drying of Foot-and-mouth Disease Virus and Storage Stability of the Infectivity of Dried Virus at 4 C.

Foot-and-mouth disease virus, type A, strain 119, propagated in cultures of calf kidney cells and in the tongue epithelium of cattle was used. The process of freeze-drying was conducted in two cycles on unit volumes of 4 ml in Pyrex ampoules, averaging 150 ampoules per run, and was studied separately from the problems of storage. Ampoules containing freeze-dried virus were flame-sealed for eith...

pH Resistance of Foot-and-Mouth Disease Virus and its Complement-Fixing Antigen

Foot-and-mouth disease virus (FMDV) vaccines

Foot-and-mouth is a disease of global socioeconomic importance, with vaccination the principle tool for control in endemic countries. Improvements in vaccines require advances in our understanding of the host immune response, one aspect of which the study group is asked to consider. Major problems limit the capacity of FMDV vaccines to control infections in developing countries. The virus is no...